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Cellprofiler custom script12/28/2023 It’s ability to have single-cell measurements is a quality which only very recently is starting to be acquired in other fields of molecular biology, and thus makes it by excellence a data-rich method. Although generally slower than other screening methods, the amount and variety of data acquired in high-throughput image-based screening is unsurpassed. Image-based screens are able to measure hundreds of cellular and sub-cellular features quantitatively and are therefore extremely powerful. In response to this, high-throughput screening of chemical compounds is a tool that is increasingly more used and in high demand due to its ability to investigate hundreds of thousands of compounds in a relatively short time. While there is increasing need for the discovery of new drugs, the number of new approved drugs per decade is in fast decline. In this small rotation project, I optimized the usage of existing computational tools for the analysis of image-based chemical screens at the single-cell level. Image-based screens, in particular, can measure hundreds of cellular features at the single-cell level and are therefore of great interest. Therefore, when not casting a hashtable literal directly to, define it with explicitly.A combination of increasing demand for new drugs and advances in automation have made high-throughput screening of chemical compounds a reality. a = 1 b = 2 c = 3 }) # !! order NOT preserved, due to (.) $ht = a = 1 b = 2 c = 3 } $ht # !! order NOT preserved In other words: When you cast a hashtable literal directly to, is implied, so the above is equivalent to: a = 1 b = 2 c = 3 } # is optionalĬaveat: This implicit ordering only applies when a hashtable literal is directly cast to, so the key/property order is not preserved in the following variations: New-Object PSCustomObject -Property a = 1 b = 2 c = 3 } # !! order NOT preserved Note how the key-definition order was preserved, even though was not specified. Note that PowerShell v3+ offers a convenient shortcut for creating a instance from a hashtable using a cast e.g.: PS> a = 1 b = 2 c = 3 } # key order is PRESERVED The source of the problem is that regular hashtables ( instances) enumerate their keys in an effectively unpredictable order (the order is an implementation detail and not guaranteed), and when you create a from a hashtable, that unpredictable key ordering is reflected in the ordering of the resulting object's properties.īy contrast, in PSv3+ you can create an ordered hashtable by placing the keyword before a hashtable literal, which results in a instance whose keys are ordered based on the order in which they were added.Ĭreating a instance from an ordered hashtable then preserves that key ordering in the resulting object's properties. Even better is to let PowerShell create the array for you, simply by capturing the output from your foreach loop in a variable ( $Table = foreach. Add() rather than using PowerShell's built-in arrays with +=, which creates a copy of the array every time. Building up a large array incrementally is more efficiently handled with a instance to which you add elements with.New-Object PSObject -property $Record could be simplified to $Record.This guarantees that the instances later created by the New-Object PSObject -property $Record calls contain properties in the same order as the keys were defined in $Record. It is, however, inefficient, because the desired column order can be ensured at the time $Record is defined, without needing an additional pipeline stage that effectively duplicates the result objects:ĭefine $Record as an ordered hashtable as follows (requires PSv3+): $Record = Name" = "" Gvee's helpful answer is a pragmatic solution that ensures that the columns appear in the desired order, because the order in which you pass property names to Select-Object is the order in which the properties are added to the resulting instances. Now I want the output formatted in this order: "SVDE","Sofie Van den Eynde","Commercial" "TVGR","Thijs Van Grimbergen","Commercial" The result: "Username","Name","Group Name" $Table | export-csv "C:\temp\SecurityGroups.csv" -NoTypeInformation $objRecord = New-Object PSObject -property $Record $Record."UserName" = $Member.samaccountname $Arrayofmembers = Get-ADGroupMember -identity $G -recursive | select name,samaccountname The code: $Groups = Get-ADGroup -Properties * -Filter * -SearchBase "OU=SERVICES,DC=XXXXXX,DC=XXXXX" Now I want to format the output of the CSV file. I'm writing a script in PowerShell that exports all security groups and their members from Active Directory.
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